Description
The Eagle Biosciences ELISA for Chemerin is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The Chemerin in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin-Peroxidase conjugated second specific anti-Chemerin-Antibody binds in turn to the immobilised Chemerin. In the closing substrate reaction the turn of the colour will be high specific catalysed, quantitatively depending on the Chemerin-level of the samples